What is meant by recognition sequence?

A recognition sequence is a DNA sequence to which a structural motif of a DNA-binding domain exhibits binding specificity. Recognition sequences are palindromes.

What is the function of recognition sequence?

First, it can be used to find genes, segments of DNA that code for a specific protein or phenotype. If a region of DNA has been sequenced, it can be screened for characteristic features of genes.

How long is a recognition sequence?

Most recognition sequences are 4–6 bp long, although they can be as large as 12 bp. The specificity of restriction enzymes means that a complete digestion will yield a reproducible array of DNA fragments.

What is a recognition sequence enzyme?

Each restriction enzyme recognizes a short, specific sequence of nucleotide bases (the four basic chemical subunits of the linear double-stranded DNA molecule—adenine, cytosine, thymine, and guanine). These regions are called recognition sequences, or recognition sites, and are randomly distributed throughout the DNA.

What is the recognition sequence for Eco r1?

EcoRI creates 4 nucleotide sticky ends with 5′ end overhangs of AATT. The nucleic acid recognition sequence where the enzyme cuts is G↓AATTC, which has a palindromic, complementary sequence of CTTAA↓G.

What is the role of ori site?

Ori: It is a genetic sequence that acts as the initiation site for replication of DNA. Any fragment of DNA, when linked to the ori region, can be initiated to replicate.

What is the recognition sequence of EcoRI restriction endonuclease?

Cleavage of DNA at an EcoRI restriction site The restriction endonuclease enzyme EcoRI recognizes the ssDNA sequence 5′-GAATTC’-3, and introduces a single-strand cut between the G & A nucleotides. This recognition site is a palindrome: the opposite strand also reads 5′-GAATTC’-3 and will be cut in the same manner.