Can you reuse tissue culture flasks?

Tissue culture flasks can be reused if they are washed and properly sterilized.

What are some of the common problems encountered when performing cell culture?

Cell culturing is complex, and it takes time to pick apart the process and isolate issues. Generally, the most common issues include abnormal growth patterns or spotty, uneven, or inconsistent cell attachment. Other problems include slow or sudden changes in growth rate or unexplainable outcomes.

Why do cell culture flasks often have vented caps?

Vented caps allow faster gas exchange without risking contamination.

How do you clean cell culture flasks?

Put flasks into the dishwasher (WEL: 2nd floor) and wash using cycle 7. This takes about 45 minutes. If necessary, place trays of flasks in an oven to dry or air dry.

What happens if seeding density is too low?

If cells are seeded at too low a density, they will go through a lag phase of growth, grow very slowly, or die out completely. If cell densities are allowed to become too high, the cells may exhaust the nutrients in the medium and die abruptly.

What are flasks coated with?

Poly-d-lysine (PDL) and collagen I coated flasks are ideal for adherent cultures of primary cells that are finicky and have difficulties attaching to the growth surface. The uniform coating creates a positive charge on the surface that helps ensure cell attachment, growth and differentiation.

What is one of the most common problems faced by researchers who use cell culture?

Contamination is a common problem in cell culture, and contaminants include mycoplasma, yeast, bacteria, and chemicals.

Why the cap of the T flask is kept little loose when placed in the incubator?

The filter cap allows exchange of CO2 in the flasks,so you can tighten the cap all the way on. Loose caps might lead to contamination.

How much media is in a T25 flask?

T25 flask received a total of 3.0 – 3.5 ml (cells + media volume needed) while a T-25 flask = 9.0 – 10 ml 11. The flask used in the split receives fresh media and is placed back in the incubator.

Can you reuse cell scrapers?

Most commercially available scrapers are disposable and therefore single use. They are routinely sterilised via irradiation by the manufacturer. These scrapers are plastic and are not normally autoclavable.

How do you know if a cell culture is contaminated?

Bacterial contamination is easily detected by visual inspection of the culture within a few days of it becoming infected;

1. Infected cultures usually appear cloudy (i.e., turbid), sometimes with a thin film on the surface.
2. Sudden drops in the pH of the culture medium is also frequently encountered.

What happens if seeding density is too high?

Seeding a high cell density than the optimum will result in cellular stress as there will be overcrowding and lack of space for cells to interact with each other and grow.

How do you increase cell density?

One approach to increase protein yield is to increase the total number of cells. In order to increase the number of cells, large bioreactors up to 25,000 liters would be used. A second approach is to increase the number of cells in the same volume, effectively increasing viable cell density.

What are cell culture flasks made of?

These flasks are made of optically clear polystyrene and have been treated for optimal cell attachment. The shape offers good pipette and cell scraper access to the corners.

What are cell culture flasks treated with?

These crystal-clear polystyrene flasks are treated with vacuum gas plasma which makes them ideal for growing cells that require an adherent surface. The cap features a .

How common is contamination cell culture?

Culture Contamination: An Overview Based on studies by the FDA, ATCC, and others, it is estimated that 5 – 30% of all cell cultures today are contaminated with mycoplasma species alone.

How do cells adhere to culture flasks?

The cell culture flasks are coated (with poly lysine) so that they have a positive charge. Now cells would have a negative charge, thereby the attraction. Also cells secrete ECM, so better adhesion to the surface.